4EVS
Crystal structure of ABC transporter from R. palustris - solute binding protein (RPA0985) in complex with 4-hydroxybenzoate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97935 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.911, 61.907, 126.267 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.780 - 1.450 |
R-factor | 0.13508 |
Rwork | 0.134 |
R-free | 0.16416 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.585 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MLPHARE |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.480 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.107 | 0.834 |
Number of reflections | 59333 | |
<I/σ(I)> | 24.5 | 2.1 |
Completeness [%] | 99.7 | 100 |
Redundancy | 6.1 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 289 | 2 M (NH4)2SO4, 0.1 M citric acid/NaOH , pH 3.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |