4EVP

Crystal Structure of Mouse Catenin beta-59 in 7.2M urea

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-10
Detector	ADSC QUANTUM 315
Wavelength(s)	1.000
Spacegroup name	C 2 2 21
Unit cell lengths	64.154, 102.441, 186.871
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.995 - 2.255
R-factor	0.1925
Rwork	0.191
R-free	0.22530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.958
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.995	2.300
High resolution limit [Å]	2.255	2.250
Rmerge	0.109	0.729
Number of reflections	29497
<I/σ(I)>	22.06	3.78
Completeness [%]	99.3	100
Redundancy	14.5	14.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			VAPOR DIFFUSION, HANGING DROP