4EVN
Crystal Structure of Fab CR6261 (somatic heavy chain with germline-reverted light chain)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.110, 159.270, 176.440 |
| Unit cell angles | 90.00, 89.73, 90.00 |
Refinement procedure
| Resolution | 55.014 - 2.851 |
| R-factor | 0.2314 |
| Rwork | 0.230 |
| R-free | 0.29280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.232 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.014 | 2.920 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Number of reflections | 86892 | |
| <I/σ(I)> | 11.27 | |
| Completeness [%] | 98.7 | 97.8 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 100 mM imidazole pH 6.5, 17.5% polyethylene glycol 8,000, and 3% 2-methyl-2,4-pentanediol, VAPOR DIFFUSION, temperature 293K |
