4EUK
Crystal structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 106.800, 106.800, 106.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.760 - 1.950 |
| R-factor | 0.17342 |
| Rwork | 0.172 |
| R-free | 0.19683 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.248 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.760 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.051 | 0.413 |
| Number of reflections | 29820 | |
| <I/σ(I)> | 23.3 | 3.9 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 13.4 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 20% PEG 550 MME, 10% PEG 20000, 0.1M MES/imidazole, 0.02M 1,6-hexanediol, 0.02M 1-butanol, 0.02M (R,S)-1,2-propandiol, 0.02M 2-propanol, 0.02M 1,3-propandiol, 0.02M 1,4-butandiol , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
