4ETY
Crystal structure of a strand-swapped dimer of Mouse Leukocyte-associated immunoglobulin-like receptor 1 (NYSGRC-006047) Extra Cellular Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-21 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 175.588, 57.427, 47.795 |
| Unit cell angles | 90.00, 105.15, 90.00 |
Refinement procedure
| Resolution | 27.810 - 1.900 |
| R-factor | 0.2017 |
| Rwork | 0.200 |
| R-free | 0.22780 |
| Structure solution method | SAD |
| Starting model (for MR) | Built using Phenix AutoSol wizard |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.450 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0025) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 36066 | |
| <I/σ(I)> | 13.6 | |
| Completeness [%] | 99.0 | 98.8 |
| Redundancy | 7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Sitting Drop Vapor Diffusion | 5.5 | 298 | Protein preparation by Refolding. Protein (11.5mg/ml in 20 mM Trizma Base pH 8.0, 100 mM NaCl; Reservoir ( 0.2 M Sodium chloride, 0.1 M BIS-TRIS pH 5.5, 25% w/v Polyethylene glycol 3,350; Index HR F10); Cryoprotection (30% Ethylene glycol in Reservior solution), Sitting Drop Vapor Diffusion, temperature 298K |
