4ETT

Crystal structure of rabbit ryanodine receptor 1 mutant E2764K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2012-02-08
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.542
Spacegroup name	P 2 21 21
Unit cell lengths	32.110, 56.244, 114.693
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.122 - 2.195
R-factor	0.2234
Rwork	0.220
R-free	0.28680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ert
RMSD bond length	0.002
RMSD bond angle	0.501
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.6_289)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.280
High resolution limit [Å]	2.195	4.740	2.200
Rmerge	0.102	0.054	0.301
Number of reflections	11079
<I/σ(I)>	8.4
Completeness [%]	98.5	98.9	91.3
Redundancy	4.3	5	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	25% PEG3350, 25% Glycerol, 0.1M HEPES, pH 7.0, vapor diffusion, hanging drop, temperature 295K