4ETT
Crystal structure of rabbit ryanodine receptor 1 mutant E2764K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-08 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 32.110, 56.244, 114.693 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.122 - 2.195 |
| R-factor | 0.2234 |
| Rwork | 0.220 |
| R-free | 0.28680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ert |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.501 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6_289) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.280 |
| High resolution limit [Å] | 2.195 | 4.740 | 2.200 |
| Rmerge | 0.102 | 0.054 | 0.301 |
| Number of reflections | 11079 | ||
| <I/σ(I)> | 8.4 | ||
| Completeness [%] | 98.5 | 98.9 | 91.3 |
| Redundancy | 4.3 | 5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 25% PEG3350, 25% Glycerol, 0.1M HEPES, pH 7.0, vapor diffusion, hanging drop, temperature 295K |
