4ETN
Crystal structure of YwlE mutant from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 84.100, 39.830, 41.330 |
| Unit cell angles | 90.00, 93.18, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.100 |
| R-factor | 0.1557 |
| Rwork | 0.155 |
| R-free | 0.17300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zgg |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.209 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.985 | 1.200 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Number of reflections | 54944 | |
| Completeness [%] | 99.0 | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.1M Tris-HCl, 0.2M lithium sulfate, 25% PEG 3350, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
