4ET9
Hen egg-white lysozyme solved from 5 fs free-electron laser pulse data
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SLAC LCLS BEAMLINE CXI |
| Synchrotron site | SLAC LCLS |
| Beamline | CXI |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2011-02-01 |
| Wavelength(s) | 1.32 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.000, 79.000, 38.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.300 - 1.900 |
| R-factor | 0.1909 |
| Rwork | 0.189 |
| R-free | 0.22650 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.025 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.300 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 9743 | |
| Completeness [%] | 98.2 | 91.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH | 293 | After crystallization, the solution used for growing the crystals (20 % NaCl, 6 % PEG 6000, 1 M Na acetate pH 3.0) was exchanged for storage solution (10 % NaCl, 1.0 M Na acetate pH 3.4), BATCH, temperature 293K |
