4ESO
Crystal structure of a putative oxidoreductase protein from Sinorhizobium meliloti 1021 in complex with NADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2012-02-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9598 |
| Spacegroup name | P 1 |
| Unit cell lengths | 62.960, 66.740, 70.540 |
| Unit cell angles | 66.50, 78.30, 62.96 |
Refinement procedure
| Resolution | 34.849 - 1.906 |
| R-factor | 0.1946 |
| Rwork | 0.193 |
| R-free | 0.23430 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.239 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 128645 | |
| Completeness [%] | 92.2 | 92.2 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2 M Ammonium Acetate, 0.1 M HEPES, 25 % PEG 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
