4ES4

Crystal structure of YdiV and FlhD complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-20
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97939
Spacegroup name	P 31 2 1
Unit cell lengths	132.486, 132.486, 145.685
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.165 - 2.900
R-factor	0.2469
Rwork	0.244
R-free	0.28260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 3TLQ 1g8e
RMSD bond length	0.011
RMSD bond angle	1.367
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.2_432))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.000
High resolution limit [Å]	2.900	6.240	2.900
Rmerge		0.050	0.748
Number of reflections	33268
<I/σ(I)>		73.13	5.03
Completeness [%]	99.8	98.3	100
Redundancy		9.9	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	293	0.1M Na/K phosphate pH5.8, 6% PEG 3000, VAPOR DIFFUSION, HANGING DROP, temperature 293K