4ERS

A Molecular Basis for Negative Regulation of the Glucagon Receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9774
Spacegroup name	P 1 21 1
Unit cell lengths	46.410, 62.250, 104.940
Unit cell angles	90.00, 93.91, 90.00

Refinement procedure

Resolution	43.456 - 2.637
R-factor	0.2335
Rwork	0.231
R-free	0.27850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.664
Data scaling software	SCALA (3.3.16)
Phasing software	PHASER
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.468	43.468	2.790
High resolution limit [Å]	2.637	8.360	2.640
Rmerge		0.044	0.391
Total number of observations		2339	9473
Number of reflections	17586
<I/σ(I)>	14.4	13.1	1.9
Completeness [%]	99.3	99.4	97.3
Redundancy	3.9	3.9	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5		0.1M Tris pH 8.5, 0.2M sodium chloride, 25% (w/v) PEG3350, VAPOR DIFFUSION