4ERL
Crystal structure of the lysine riboswitch bound to a lysine-glycine dipeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2010-04-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 |
| Unit cell lengths | 119.878, 119.878, 58.743 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.620 - 3.000 |
| R-factor | 0.1974 |
| Rwork | 0.195 |
| R-free | 0.23380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3D0U) |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.933 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.620 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.113 | 0.321 |
| <I/σ(I)> | 5.1 | 1.9 |
| Completeness [%] | 97.8 | 96.5 |
| Redundancy | 1.92 | 1.93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 303 | 10 mM Na-HEPES pH 7.0, 2 M Li2SO4, and 5 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
