4ERD
Crystal structure of the C-terminal domain of Tetrahymena telomerase protein p65 in complex with stem IV of telomerase RNA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792,0.9794,0.9718 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 88.830, 88.830, 119.210 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 64.638 - 2.589 |
| R-factor | 0.2221 |
| Rwork | 0.220 |
| R-free | 0.27170 |
| Structure solution method | MAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.496 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | HKL2Map |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.929 | 2.670 |
| High resolution limit [Å] | 2.588 | 2.588 |
| Number of reflections | 16940 | |
| <I/σ(I)> | 16.18 | 2.57 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.05 M sodium cacodylate, 0.04 M magnesium chloride, 5% v/v MPD, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
