4ERD

Crystal structure of the C-terminal domain of Tetrahymena telomerase protein p65 in complex with stem IV of telomerase RNA

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792,0.9794,0.9718
Spacegroup name	P 31 2 1
Unit cell lengths	88.830, 88.830, 119.210
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	64.638 - 2.589
R-factor	0.2221
Rwork	0.220
R-free	0.27170
Structure solution method	MAD
RMSD bond length	0.002
RMSD bond angle	0.496
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	HKL2Map
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.929	2.670
High resolution limit [Å]	2.588	2.588
Number of reflections	16940
<I/σ(I)>	16.18	2.57
Completeness [%]	99.9	99.9
Redundancy	7.48

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	0.05 M sodium cacodylate, 0.04 M magnesium chloride, 5% v/v MPD, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K