4EQX
Crystal Structure of the C43S Mutant of Staphylococcus aureus CoADR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2008-12-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.960, 65.380, 94.470 |
| Unit cell angles | 90.00, 104.83, 90.00 |
Refinement procedure
| Resolution | 48.850 - 1.700 |
| R-factor | 0.1954 |
| Rwork | 0.193 |
| R-free | 0.23250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | d*TREK (9.2L) |
| Data scaling software | d*TREK (9.2L) |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 53.160 | 53.160 | 1.760 |
| High resolution limit [Å] | 1.700 | 3.660 | 1.700 |
| Rmerge | 0.071 | 0.038 | 0.360 |
| Total number of observations | 67430 | 42172 | |
| Number of reflections | 98511 | ||
| <I/σ(I)> | 11 | 30.6 | 3.1 |
| Completeness [%] | 99.9 | 99.7 | 99.9 |
| Redundancy | 6.3 | 6.59 | 4.27 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | PEG 400, magnesium chloride, HEPES, NADP+, pH 7.2, vapor diffusion, sitting drop, temperature 293K, VAPOR DIFFUSION, SITTING DROP |
