4EQI
Crystal structure of serratia fonticola carbapenemase SFC-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.117 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.330, 85.430, 70.280 |
| Unit cell angles | 90.00, 103.27, 90.00 |
Refinement procedure
| Resolution | 17.850 - 1.380 |
| R-factor | 0.141 |
| Rwork | 0.140 |
| R-free | 0.18800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ov5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.029 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.430 |
| High resolution limit [Å] | 1.380 | 2.970 | 1.380 |
| Rmerge | 0.064 | 0.023 | 0.568 |
| Number of reflections | 99419 | ||
| <I/σ(I)> | 18.234 | 46.03 | 2.03 |
| Completeness [%] | 99.6 | 99.4 | 99.4 |
| Redundancy | 3.6 | 3.8 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Na acetate, PEG 3350, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
