4EQ2
Crystal Structure Analysis of Chicken Interferon Gamma Receptor Alpha Chain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 63.536, 63.536, 216.323 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.570 - 2.502 |
| R-factor | 0.2203 |
| Rwork | 0.218 |
| R-free | 0.26640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4eq3 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.811 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.105 | 0.396 |
| Number of reflections | 9650 | |
| <I/σ(I)> | 35.373 | 4.685 |
| Completeness [%] | 99.3 | 93.7 |
| Redundancy | 23.6 | 16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 20% (v/v) polyethylene glycol 5000 monomethyl ether, 0.1M HEPES, 0.5M sodium carbonate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
