4EQ1
Crystal Structure of the ARNT PAS-B homodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97904 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.315, 61.678, 55.514 |
| Unit cell angles | 90.00, 124.60, 90.00 |
Refinement procedure
| Resolution | 24.047 - 1.600 |
| R-factor | 0.2028 |
| Rwork | 0.202 |
| R-free | 0.22650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3F1P chain A |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.448 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 24.047 |
| High resolution limit [Å] | 1.600 |
| Rmerge | 0.047 |
| Number of reflections | 34166 |
| <I/σ(I)> | 38.8 |
| Completeness [%] | 99.7 |
| Redundancy | 4.55 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 2M Ammonium Sulfate, 3% PEG400, 1% PEI, 50mM Tris pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
