4EPS

Crystal structure of a fimbrial protein (BACOVA_04982) from Bacteroides ovatus ATCC 8483 at 1.85 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-03-10
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 41 21 2
Unit cell lengths	77.956, 77.956, 250.498
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.056 - 1.850
R-factor	0.1647
Rwork	0.164
R-free	0.17990
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	0.970
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.056	29.056	1.900
High resolution limit [Å]	1.850	8.270	1.850
Rmerge		0.079	0.993
Total number of observations		6822	42008
Number of reflections	67064
<I/σ(I)>	10.5	20.1	2.4
Completeness [%]	99.9	97.2	100
Redundancy	8.7	7.7	8.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	1.6M ammonium sulfate, 0.1M MES pH 6.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K