4EPM
Crystal Structure of Arabidopsis GH3.12 (PBS3) in Complex with AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2011-03-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 59.765, 65.769, 154.972 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.532 - 2.099 |
| R-factor | 0.2017 |
| Rwork | 0.200 |
| R-free | 0.24030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.036 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.500 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 35574 | |
| <I/σ(I)> | 13.2 | 2 |
| Completeness [%] | 97.5 | 95.9 |
| Redundancy | 4.9 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 2 M Ammonium Sulfate, .1 M PIPES, 2% PEG-400, 5 mM AMP, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
