4EPL
Crystal Structure of Arabidopsis thaliana GH3.11 (JAR1) in Complex with JA-Ile
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 76 |
| Detector technology | CCD |
| Collection date | 2011-07-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 107.039, 159.024, 76.401 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.502 - 2.007 |
| R-factor | 0.171 |
| Rwork | 0.169 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.025 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.500 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 43730 | |
| <I/σ(I)> | 26.2 | 2.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.3 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 1.5 M tartrate, 0.1 M MOPSO, 5 mM ATP, 5 mM MgCl2, 5mM jasmonic acid, 5 mM isoleucine, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
