4EPH
CRYSTAL STRUCTURE OF RAT CARNITINE PALMITOYLTRANSFERASE 2 IN COMPLEX with CoA-site inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2005-12-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979650 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 67.640, 67.640, 309.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.310 - 2.300 |
| R-factor | 0.18888 |
| Rwork | 0.186 |
| R-free | 0.24910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2deb |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.509 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.310 | 2.410 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Completeness [%] | 98.8 | 92.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | Hampton Index #69 0.2 M Ammonium sulfate, 0.1 M Tris 8.5, 25 % (w/v)polyethylene glycol 3,350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
