4EO5
Yeast Asf1 bound to H3/H4G94P mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-08 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 97.691, 97.691, 115.072 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.350 |
| R-factor | 0.20597 |
| Rwork | 0.204 |
| R-free | 0.24027 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hue |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.282 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.240 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.090 | 0.474 |
| Number of reflections | 28711 | |
| <I/σ(I)> | 8.4 | 2.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.14 | 5.18 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 298 | 7.14% PEG 3350, 0.2 M Magnesium Acetate, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
