4EO2

Structure of the bacteriophage C1 tail knob protein, gp12

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-17
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00931, 1.00579, 1.00939
Spacegroup name	P 21 21 2
Unit cell lengths	205.614, 209.640, 102.975
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.002 - 3.007
R-factor	0.2406
Rwork	0.238
R-free	0.28240
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.860
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXCD
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	146.794	50.000	3.110
High resolution limit [Å]	3.007	6.460	3.007
Rmerge	0.068		0.425
Number of reflections	87794
<I/σ(I)>	27.44		3.74
Completeness [%]	98.8	99.5	91
Redundancy	7.2		5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	293	30% MPD, 0.1 M sodium citrate, pH 6.0, EVAPORATION, temperature 293K