4EO1
crystal structure of the TolA binding domain from the filamentous phage IKe
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-07-22 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 28.550, 33.810, 65.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.160 - 1.800 |
| R-factor | 0.1896 |
| Rwork | 0.187 |
| R-free | 0.21880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x9b |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.340 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.200 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 11481 | |
| Completeness [%] | 97.1 | 96.5 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 2 M (NH4)2SO4, 0.1 M Tris 8.5, 5% PEG400, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
