4EO0
crystal structure of the pilus binding domain of the filamentous phage IKe
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-09-11 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 59.300, 59.300, 137.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.940 - 1.610 |
| R-factor | 0.1884 |
| Rwork | 0.187 |
| R-free | 0.20740 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.040 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.940 | 1.720 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmerge | 0.090 | 0.280 |
| Number of reflections | 16254 | |
| Completeness [%] | 99.7 | 78.8 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 15-20% PPG400 0.1 M Bis-Tris 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
