4ENL
CRYSTAL STRUCTURE OF HOLOENZYME REFINED AT 1.9 ANGSTROMS RESOLUTION: TRIGONAL-BIPYRAMIDAL GEOMETRY OF THE CATION BINDING SITE
Experimental procedure
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 124.100, 124.100, 66.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 * - 1.900 |
| R-factor | 0.149 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 3.200 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.900 * |
| Rmerge | 0.060 * |
| Total number of observations | 59713 * |
| Number of reflections | 29393 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enolase | 2 (%) | |
| 2 | 1 | drop | Mg2+ | 2 (mM) | |
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | ammonium sulfate | 27 (%sat) | |
| 6 | 1 | drop | sodium citrate | 50 (mM) | |
| 7 | 1 | reservoir | ammonium sulfate | 55 (%sat) |
