4ENE

Structure of the N- and C-terminal trimmed ClC-ec1 Cl-/H+ antiporter and Fab Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	232.260, 97.520, 173.960
Unit cell angles	90.00, 132.87, 90.00

Refinement procedure

Resolution	24.866 - 2.400
R-factor	0.2414
Rwork	0.239
R-free	0.27830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.188
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.400
Rmerge	0.095
Number of reflections	108884
Completeness [%]	98.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	295	27% peg400, 100mM Ca-acetate, 100mM tris-HCl, pH 8.5, VAPOR DIFFUSION, temperature 295K