4ENC

Crystal structure of fluoride riboswitch

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 2
Unit cell lengths	57.010, 75.223, 42.905
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.505 - 2.272
R-factor	0.2216
Rwork	0.220
R-free	0.25150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4enb
RMSD bond length	0.026
RMSD bond angle	0.780
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.272
Rmerge	0.073
Number of reflections	8726
<I/σ(I)>	31
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	0.1 M MES sodium, pH 6.5, 35-40% w/v MPD, VAPOR DIFFUSION, temperature 293K