4EM8
The Structure of Ribose 5-phosphate Isomerase B from Anaplasma phagocytophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-06 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.360, 62.490, 73.960 |
| Unit cell angles | 90.00, 93.13, 90.00 |
Refinement procedure
| Resolution | 35.307 - 1.950 |
| R-factor | 0.1814 |
| Rwork | 0.179 |
| R-free | 0.22030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o1x |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.421 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.307 | 2.000 | |
| High resolution limit [Å] | 1.950 | 8.720 | 1.950 |
| Rmerge | 0.048 | 0.021 | 0.399 |
| Number of reflections | 23547 | 281 | 1715 |
| <I/σ(I)> | 21.99 | 55.99 | 3.98 |
| Completeness [%] | 99.8 | 98.3 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | AnphA.00455.a.A1 PW 35203 at 45.59 mg/mL, 200 mM potassium formate, 20% PEG3350, cryoprotectant: 20% ethylene glycol , pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
