4EM1
staphylococcus aureus MarR native
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-24 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 36.122, 75.218, 109.427 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.000 - 3.000 |
| Rwork | 0.269 |
| R-free | 0.29350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4em2 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 | 3.030 |
| High resolution limit [Å] | 3.000 | 2.900 |
| Rmerge | 0.065 | 0.403 |
| Number of reflections | 3179 | |
| <I/σ(I)> | 16.8 | 3.6 |
| Completeness [%] | 98.3 | 99.3 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 20% 5000 MME, 0.2M LiSO4, 0.1M Tris PH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
