Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.073, 90.833, 58.130 |
| Unit cell angles | 90.00, 102.59, 90.00 |
Refinement procedure
| Resolution | 24.624 - 1.932 |
| R-factor | 0.1871 |
| Rwork | 0.185 |
| R-free | 0.21670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3JSC AND 1X75 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.066 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.000 | 2.020 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.050 | 0.247 |
| Number of reflections | 48855 | |
| <I/σ(I)> | 19 | 9.12 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.99 | 8.99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 50MM MES, 1.6M (NH4)2SO4, 50MM MGCL2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
