4ELO

Ferric binding protein in apo form 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-01
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	42.124, 139.349, 326.337
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.148 - 1.914
R-factor	0.1656
Rwork	0.163
R-free	0.20730
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.031
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_596))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.910
Number of reflections	143194
Completeness [%]	96.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	0.1M ammonium acetate, 30% PEG 4000, 0.1M Sodium acetate pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K