4ELJ

Crystal structure of the inactive retinoblastoma protein phosphorylated at T373

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-04
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	51.620, 129.510, 135.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.390 - 2.700
R-factor	0.2165
Rwork	0.212
R-free	0.26610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.870
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.390	2.850
High resolution limit [Å]	2.700	2.700
Number of reflections	23387
Completeness [%]	92.3	90

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277.15	100mM HEPES, 100mM Ammonium Fluoride, 16% PEG4K, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K