4ELD
Crystal Structure of an Activated Variant of Small Heat Shock Protein Hsp16.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-30 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97945 |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 184.583, 184.583, 184.583 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.332 - 2.701 |
| R-factor | 0.2161 |
| Rwork | 0.214 |
| R-free | 0.23670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1shs |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.089 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.043 | 0.600 |
| Number of reflections | 14902 | |
| <I/σ(I)> | 29.6 | 2.1 |
| Completeness [%] | 98.9 | 100 |
| Redundancy | 4.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 1.8 M ammonium sulfate, 20 mM Tris, pH 8.0, 10% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
