4EL6

Crystal structure of IPSE/alpha-1 from Schistosoma mansoni eggs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9762
Spacegroup name	P 61
Unit cell lengths	54.388, 54.388, 56.712
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.110 - 1.710
R-factor	0.15485
Rwork	0.154
R-free	0.17693
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.020
RMSD bond angle	1.955
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.6.0119)

Data quality characteristics

	Overall
Low resolution limit [Å]	47.110
High resolution limit [Å]	1.710
Number of reflections	10326
Completeness [%]	99.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	30 mM NaF, 15% 2-propanol, 8% PEG 1000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K