4EL5

Crystal structure of GPb in complex with DK12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	293
Detector technology	CCD
Collection date	2011-11-16
Detector	MAR CCD 165 mm
Wavelength(s)	0.8
Spacegroup name	P 43 21 2
Unit cell lengths	128.880, 128.880, 116.652
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.800 - 2.000
R-factor	0.1656
Rwork	0.163
R-free	0.20990
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3sym
RMSD bond length	0.024
RMSD bond angle	1.872
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	REFMAC
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	86.490	2.030
High resolution limit [Å]	2.000	2.000
Number of reflections	55243
<I/σ(I)>	7.2	1.3
Completeness [%]	87.6	50.2
Redundancy	3.6	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	6.7	289	10mM BES buffer, pH 6.7, SMALL TUBES, temperature 289K