4EKA

Final Thaumatin Structure for Radiation Damage Experiment at 25 K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	25
Detector technology	CCD
Collection date	2010-06-22
Detector	ADSC QUANTUM 270
Wavelength(s)	0.917
Spacegroup name	P 41 21 2
Unit cell lengths	57.350, 57.350, 150.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.940 - 1.550
R-factor	0.1913
Rwork	0.191
R-free	0.20040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.160
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	BUSTER (2.8.0)
Refinement software	BUSTER-TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	21.940
High resolution limit [Å]	1.550
Number of reflections	37321

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	298	sodium potassium tartrate, pH 6.8, vapor diffusion, hanging drop, temperature 298K