4EJZ
Structure of MBOgg1 in complex with low affinity DNA ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-18 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.0089 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.805, 103.596, 66.725 |
| Unit cell angles | 90.00, 90.65, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.050 |
| R-factor | 0.25282 |
| Rwork | 0.251 |
| R-free | 0.29296 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ejy |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.869 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.160 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.125 | 0.375 |
| Number of reflections | 14731 | |
| Completeness [%] | 99.0 | 93.8 |
| Redundancy | 7 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 20% PEG 3500, 0.1-0.15M KH2PO4, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
