4EJY
Structure of MBOgg1 in complex with high affinity DNA ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-30 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.0089 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.613, 102.884, 67.284 |
| Unit cell angles | 90.00, 90.18, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.22417 |
| Rwork | 0.222 |
| R-free | 0.25791 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3i0w |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.986 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.065 | 0.321 |
| Number of reflections | 52075 | |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 7.5 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 289 | 20% PEG 3350, 0.2-0.25M Sodium Malonate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
