4EJO

Crystal structure of padr family transcriptional regulator from Eggerthella lenta DSM 2243

Replaces:  3RI2

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97948, 0.97959
Spacegroup name	I 41
Unit cell lengths	55.783, 55.783, 132.425
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.100
R-factor	0.1833
Rwork	0.181
R-free	0.23530
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.667
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.120
High resolution limit [Å]	2.100	2.100
Rmerge	0.126	0.542
Number of reflections	11880
<I/σ(I)>	33.9	4
Completeness [%]	100.0	100
Redundancy	9.6	8.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	277	0.1M PHOSPHATE-CITRATE PH 4.2, 40% ETHANOL, 5% PEG 1000, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE, temperature 277 K