4EJE

Structure Of The Tsg101 UEV Domain In Complex With an Ebola PTAP late Domain Peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM16
Synchrotron site	ESRF
Beamline	BM16
Temperature [K]	298
Detector technology	CCD
Collection date	2008-03-03
Detector	MAR CCD 165 mm
Wavelength(s)	0.97
Spacegroup name	I 4
Unit cell lengths	105.544, 105.544, 75.304
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.000 - 2.200
R-factor	0.21729
Rwork	0.211
R-free	0.27443
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2f0r
RMSD bond length	0.014
RMSD bond angle	2.086
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.16)
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	74.631	19.997	2.320
High resolution limit [Å]	2.199	6.960	2.199
Rmerge	0.059	0.035	0.404
Number of reflections	21003
<I/σ(I)>	19.7	18	1.7
Completeness [%]	99.7	90.3	100
Redundancy	8.2	6.1	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	278	0.1 M of ammonium sulphate, 25% PEG 4K, 25% 0.1 M Hepes, pH 7.0, vapor diffusion, hanging drop, temperature 278K