4EIW
Whole cytosolic region of atp-dependent metalloprotease FtsH (G399L)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 146.153, 146.153, 349.064 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 71.530 - 3.900 |
| R-factor | 0.29999 |
| Rwork | 0.299 |
| R-free | 0.31154 |
| Structure solution method | MIR |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.895 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 4.100 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Number of reflections | 37156 | |
| Completeness [%] | 97.1 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 0.1M TRIS-HCL, 1% PEG 8000, 0.2mM ZN ACETATE, 2.5MM ADP, 5mM MgCl2, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
