4EIR
Structural basis for substrate targeting and catalysis by fungal polysaccharide monooxygenases (PMO-2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.170, 41.990, 69.250 |
| Unit cell angles | 90.00, 97.94, 90.00 |
Refinement procedure
| Resolution | 22.860 - 1.100 |
| R-factor | 0.133 |
| Rwork | 0.132 |
| R-free | 0.14900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eja |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.342 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 22.860 |
| High resolution limit [Å] | 1.100 |
| Number of reflections | 155130 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 295 | pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
