4EIE
Crystal structure of cytochrome c6C from Synechococcus sp. PCC 7002
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-07-13 |
| Detector | MAR555 FLAT PANEL |
| Wavelength(s) | 0.8149 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 56.130, 56.130, 49.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.840 - 1.030 |
| R-factor | 0.13671 |
| Rwork | 0.136 |
| R-free | 0.16344 |
| Structure solution method | SAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.898 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.040 | 1.090 |
| High resolution limit [Å] | 1.030 | 1.030 |
| Rmerge | 0.088 | 0.832 |
| Number of reflections | 39532 | |
| <I/σ(I)> | 21.8 | 2.9 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 23.4 | 13.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | Protein solution: 15 mg/ml cytochrome c6C in 0.1 M TRIS pH 7.5, 0.2 M NaCl and 1 mM PMSF; Precipitant solution: 2 M NaCl and 2 M (NH4)2SO4. pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
