4EID
Crystal structure of cytochrome c6 Q57V mutant from Synechococcus sp. PCC 7002
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-07-13 |
| Detector | MAR555 FLAT PANEL |
| Wavelength(s) | 0.8166 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.781, 27.637, 44.099 |
| Unit cell angles | 90.00, 101.16, 90.00 |
Refinement procedure
| Resolution | 43.260 - 1.130 |
| R-factor | 0.13931 |
| Rwork | 0.138 |
| R-free | 0.17574 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dr0 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.872 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.200 |
| High resolution limit [Å] | 1.130 | 1.130 |
| Rmerge | 0.050 | 0.451 |
| Number of reflections | 28144 | |
| <I/σ(I)> | 15.3 | 2 |
| Completeness [%] | 99.1 | 96.8 |
| Redundancy | 3.3 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 2.2M Ammonium sulphate, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
