4EIC
Crystal structure of reduced cytochrome c6 from Synechococcus sp. PCC 7002 at ultra-high resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-14 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8166 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.860, 27.690, 44.070 |
| Unit cell angles | 90.00, 101.10, 90.00 |
Refinement procedure
| Resolution | 32.550 - 0.840 |
| R-factor | 0.1058 |
| Rwork | 0.106 |
| R-free | 0.12710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dr0 |
| RMSD bond length | 0.026 |
| RMSD bond angle | 0.057 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.550 | 0.860 |
| High resolution limit [Å] | 0.840 | 0.840 |
| Rmerge | 0.063 | 0.507 |
| Number of reflections | 64908 | |
| <I/σ(I)> | 15.21 | 3.23 |
| Completeness [%] | 94.8 | 80.6 |
| Redundancy | 6 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 2.2M Ammonium sulphate, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
