4EIB
Crystal Structure of Circular Permuted CBM21 (CP90) Gives Insight into the Altered Selectivity on Carbohydrate Binding.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-14 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97622 |
| Spacegroup name | P 4 |
| Unit cell lengths | 66.945, 66.945, 44.356 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.820 - 1.860 |
| R-factor | 0.15967 |
| Rwork | 0.151 |
| R-free | 0.23560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v8l |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.903 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.940 | 1.890 |
| High resolution limit [Å] | 1.829 | 1.860 |
| Rmerge | 0.038 | 0.038 |
| Number of reflections | 14988 | |
| <I/σ(I)> | 49.4 | 13.69 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.5 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 1.26M (NH4)2SO4, 0.1M acetate pH 4.5, 0.2M NaCl , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
