4EI9
Crystal structure of Bacillus cereus TubZ, GTP-form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2011-06-28 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.501, 76.443, 97.628 |
| Unit cell angles | 90.00, 105.22, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.300 |
| Rwork | 0.206 |
| R-free | 0.29440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.360 |
| High resolution limit [Å] | 3.300 | 8.940 | 3.300 |
| Rmerge | 0.082 | 0.033 | 0.377 |
| Number of reflections | 10758 | ||
| <I/σ(I)> | 10.4 | ||
| Completeness [%] | 99.8 | 96.3 | 100 |
| Redundancy | 7 | 6.5 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 50% PEG 400, 0.1M MES, 1mM GTPgammaS, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
