4EGV
Crystal structure of a monomeric SCP2-thiolase like protein type 1 (STLP1) from Mycobacterium smegmatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-11-26 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 1 |
| Unit cell lengths | 96.308, 102.004, 102.423 |
| Unit cell angles | 116.46, 101.28, 97.44 |
Refinement procedure
| Resolution | 47.950 - 2.710 |
| R-factor | 0.23492 |
| Rwork | 0.234 |
| R-free | 0.26107 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.872 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 | 2.640 |
| High resolution limit [Å] | 2.700 | 2.700 | 2.600 |
| Rmerge | 0.090 | 0.400 | 0.300 |
| Number of reflections | 83286 | ||
| <I/σ(I)> | 4.6 | 2.3 | |
| Redundancy | 4.1 | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 291 | 100mM HEPES pH 7.5, 20% PEG 4000, 10% isopropanol, Microbatch, temperature 291K |
