4EGL
Crystal structure of two tandem RNA recognition motifs of Human antigen R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-04-21 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.99985 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 41.181, 132.724, 31.102 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.330 - 2.900 |
| R-factor | 0.26458 |
| Rwork | 0.263 |
| R-free | 0.29440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fxl |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.250 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.465 | |
| Number of reflections | 4062 | |
| <I/σ(I)> | 2.26 | |
| Completeness [%] | 97.3 | 92.9 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 287 | 1.5M Lithium sulfate monohydrate, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 287K |
